INDIRECT SPECTROPHOTOMETRIC DETERMINATION OF IBUPROFEN USING CRYSTAL VIOLET DYE IN THE PRESENCE OF N-BROMOSUCCINIMIDE AS AN OXIDIZING AGENT

Nada H. Hamad; Abeer Qais Younes; Mohammed Salim Sheet Al-Enizzi

Авторы

Nada H. Hamad Department of Chemistry, College of Education for Women, University of Mosul, Mosul, Iraq
Abeer Qais Younes Department of Chemistry, College of Education for Women, University of Mosul, Mosul, Iraq
Mohammed Salim Sheet Al-Enizzi Environmental Research Center, University of Mosul, Iraq

Ключевые слова:

Ibuprofen, Crystal Violet, N-Bromosuccinimide, Dye-Bleaching Reaction

Аннотация

Ibuprofen in pure material and in pharmaceutical dosage forms was quantified by an indirect spectrophotometric procedure designed for simplicity, sensitivity, and accuracy. The analytical sequence begins with oxidation of ibuprofen by N-bromosuccinimide (NBS). Any NBS remaining after this step reacts with a fixed amount of crystal violet in hydrochloric acid and bleaches the dye. The loss in crystal-violet absorbance was monitored at 630 nm; its magnitude changed directly with the amount of ibuprofen present. Once the optimal reaction parameters were determined, the calibration response was linear over the range of 1.0–20.0 μg mL−1 of ibuprofen. The value of molar absorptivity obtained was 1.05 × 10⁴ L mol⁻¹ cm⁻¹, which was found to be the most sensitive procedure. The detection limit and the quantification limit are 0.0797 μg mL-1 and 0.2658 μg mL-1, respectively, to be determined experimentally, and the accuracy and precision are 101.13% and 0.17%, respectively. The procedure was applied to marketed ibuprofen tablets of different manufacturers, satisfactory analytical performance was obtained.

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